Publications

2005 Khoo, K.M., Chang, C.F., Schubert, J., Wondrak, E.M., Chng, H.H. Expression and purification of the recombinant His-tagged GST-CD38 fusion protein using the baculovirus/insect cell expression system. Protein Expression & Purification 40, 396-403.
2000 Geselowitz, D.A., Cramer, H., Wondrak, E.M., Player, M.R. and Torrence, P.F. Fluorescence resonance energy transfer analysis of RNase L-catalyzed oligonucleotide cleavage. Antisense Nucleic Acid Drug Dev. 10, 45-51.
1999 Louis, J.M., Wondrak, E.M., Kimmel, A.R., Wingfield, P.T. and Nashed, N.T. Proteolytic Processing of
HIV-1 Protease Precursor, Kinetics and Mechanism. J. Biol. Chem. 274, 23437-23442.
1998 Player, M.R., Wondrak, E.M., Bayly, S.F. and Torrence, P.F. Ribonuclease L, a 2-5A-Dependent Enzyme: Purification to Homogeneity and Assays for 2-5A Binding and Catalytic Activity. Methods 15, 243-253.
1997 Weber, I.T., Wu, J., Adomat, J., Harrison, R.W., Kimmel, A.R., Wondrak, E.M. and Louis, J.M. Crystallographic Analysis of Human Immunodeficiency Virus 1 Protease with an Analog of the Conserved CA-p2 Substrate -- Interactions with Frequently Occurring Glutamic Acid Residue at P2' Position of Substrates. Eur. J. Biochem. 249, 523-530.
1996 Wondrak, E.M. and Louis, J.M. Influence of Flanking Sequences on Dimer Stability of the Human Immunodeficiency Virus Type 1 Protease. Biochemistry 35, 12957-12962.
1996 Wondrak, E.M., Nashed, N.T., Haber, M.T., Jerina, D.M., and Louis, J.M. A Transient Precursor of the HIV-1 Protease: Isolation, Characterization and Kinetics of Maturation. J. Biol. Chem. 271, 4477-4481.
1995 Louis, J.M., Wondrak, E.M., and Nashed, N.T. Proteolytic Processing Mechanism of Human Immunodeficiency Virus. AIDS Res. Hum. Retroviruses 11s, S162.
1994 Wondrak, E.M., Kimmel, A.R., and Louis, J.M. Processing of the Mature HIV-1 Nucleocapsid Protein by the Viral Protease. J. Prot. Chem. 13, 543.
1994 Wondrak, E.M., Sakaguchi, K., Rice, W.G., Kun, E., Kimmel, A.R., and Louis, J.M. Removal of Zinc is Required for Processing of the Mature Nucleocapsid Protein of Human Immunodeficiency Virus, Type 1, by the Viral Protease. J. Biol. Chem. 269, 21948-21950.
1993 Wondrak, E.M., Louis, J.M., de Rocquigny, H., Chermann, J.C., and Roques, B.P. The gag Precursor Contains a Specific HIV-1 Protease Cleavage Site Between the NC (p7) and p1 Proteins. FEBS Lett. 333, 21-24.
1993 Camplo, M., Charvet, A.-S., Faury, P., Wondrak, E.M., Chermann, J.-C., and Kraus, J.-L. Synthesis and Antiviral Activity of a Potential Prodrug: N4-retinol 3'-thia-2',3'-dideoxycytidine. Med. Chem. Res. 3, 87-95.
1991 Louis, J.M., McDonald, R.A., Nashed, N.T., Wondrak, E.M., Jerina, D.M., Oroszlan, S., and Mora, P. Autoprocessing of the HIV-1 Protease Using Purified Wild-Type and Mutated Fusion Proteins Expressed at High Levels in Escherichia coli. Eur. J. Biochem. 199, 361-369.
1991 Tozser, J., Blaha, I., Copeland, T.D., Wondrak, E.M., and Oroszlan, S. Comparison of the HIV-1 and HIV-2 Proteinases Using Oligopeptide Substrates Representing Cleavage Sites in gag and gag-pol Polyproteins. FEBS Lett. 281, 77-80.
1991 Wondrak, E.M., Louis, J.M., Mora, P.T., and Oroszlan, S. Purification of the HIV-1 Wild-Type Protease and Characterization of Proteolytically Inactive HIV-1 Protease Mutants By Pepstatin A Chromatography. FEBS Lett. 280, 347-350.
1991 Wondrak, E.M., Louis, J.M., and Oroszlan, S. The Effect of Salt on the Michaelis Menten Constant of the HIV-1 Protease Correlates with the Hofmeister Series. FEBS Lett. 280, 344-346.
1991 Tozser, J., Gustchina A., Weber, I.T., Blaha, I., Wondrak, E.M., and Oroszlan, S. Studies on the Role of the S4 Substrate Binding Site of HIV Proteases. FEBS Lett. 279, 356-360.
1990 Wondrak, E.M., Copeland, T.D., Louis, J.M., and Oroszlan, S. A Solid Phase Assay for the Protease of the Human Immunodeficiency Virus. Anal. Biochem. 188, 82-85.
1990 Grobelny, D., Wondrak, E.M., Galardy, R.E., and Oroszlan, S. Selective Phosphinate Transition State Analogue Inhibitors of the Human Immunodeficiency Virus. Biochem. Biophys. Res. Commun. 169, 1111-1116.
1990 Copeland, T.D., Wondrak, E.M., Tozser, J., Roberts, M.M., and Oroszlan, S. Substitution of Proline with Pipecolic Acid at the Scissile Bond Converts a Peptide Substrate of HIV Proteinase into a Selective Inhibitor. Biochem. Biopphys. Res. Commun. 169, 310-314.
1989 Louis, J.M., Wondrak, E.M., Smith, C.A.D., Mora, P.T., and Oroszlan, S. E.coli expressed HIV-1 Protease Mutants Containing Arg87/Asn88 Substitutions are Inactive. In: Curr. Comm. Mol. Biol. - Viral Proteinases as Targets for Chemotherapy. H. Krausslich, S. Oroszlan and E. Wimmer (eds). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 161-167.
1989 Louis, J.M., Smith, C.A.D., Wondrak, E.M., Mora, P.T., and Oroszlan, S. Substitution Mutations of the Highly Conserved Arginine 87 of HIV-1 Protease Results in Loss of Proteolytic Activity. Biochem. Biophys. Res. Commun. 164, 30-38.
1989 Nashed, N.T., Louis, J.M., Sayer, J.M., Wondrak, E.M., Mora, P., Oroszlan, S., and Jerina, D.M. Continuous Spectrophotometric Assay for Retroviral Proteases of HIV-1 and AMV. Biochem. Biophys. Res. Commun. 163, 1079-1085.
1989 Louis, J.M., Wondrak, E.M., Copeland, T.D., Smith, C.A.D., Mora, P.T., and Oroszlan, S. Chemical Synthesis and Expression of the HIV-1 Protease in E. coli. Biochem. Biophys. Res. Commun. 159, 87-94.
1988 Wondrak, E.M., Löwer, J., and Kurth, R. Inhibition of HIV-1 RNA Dependent DNA Polymerase and Cellular Polymerases alpha, beta and gamma by Phosphono ormic Acid and Other Drugs. Chemical Abstracts 21, 23.
1988 Wondrak, E.M., Löwer, J., and Kurth, R. Inhibition of HIV-1 RNA Dependent DNA Polymerase and Cellular Polymerases alpha, beta and gamma by Phosphono Formic Acid and Other Drugs. J. Antimicrob. Chemothera. 21, 151-161.
1987 Wondrak, E.M., Behr, E., Löwer, J., and Kurth, R. Wirkung von Foscarnet-Derivaten auf die enzymatische Aktivität der Reversen Transkriptase von HIV-1 und der zellulären DNS Polymerasen alpha, beta und gamma. In: AIDS, Statusseminar des Bundesministeriums für Forschung und Technologie, Bonn-Bad Godesberg, September 1987, W. Vettermann und M. Schauzu (eds). Berlin: Bundesgesundheitsamt, pp. 321-332.
1987 Löwer, J., Wondrak, E.M., and Kurth, R. Genome Analysis and Reverse Transcriptase Activity of Human Teratocarcinoma-derived Retrovirus. J. gen. Virol. 68, 2807-2815.
1986 Wondrak, E.M., Löwer, J., and Kurth, R. Functional Purification and Enzymatic Characterization of RNA Dependent DNA Polymerase of HIV. J. gen. Virol. 67, 2791-2797.
1986 Werner, A., Wondrak, E.M., and Kurth, R. Ansätze zur Chemotherapie und Immunprophylaxe (Impfung) der LAV/HTLV-III Infektionen. In: AIDS II, Jahrestagung der Deutschen Gesellschaft für Infektiologie, Mai 1986, E.B. Helm, W. Stille und E. Vanek (eds). München: Zuckschwertverlag, pp. 207-213.
1986 Kurth, R., Wondrak, E.M., and Löwer, J. Aspekte der Chemotherapie von LAV/HTLV-III-Infektionen. In: Prophylaxe und Therapie der Viruserkrankungen. Bericht von der Tagung der Deutschen Vereinigung zur Bekämpfung der Viruserkrankungen e.V., München 1986, F. Deinhardt und H. Spiess (eds). Marburg/Lahn: Die Medizinische Verlagsgesellschaft mbH, pp. 80-88.

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